Use of biodegradable polyester-based microvessels for micropropagation of mature Eucalyptus microcorys

Background: Micropropagation, an in vitro vegetative propagation technique using small propagules is one of the main applications plant tissue culture. It can be used to clone specific plants with desired traits and reduce cost propagation. In this study, we developed a protocol for micropropagation Eucalyptus microcorys F.Muell selected mature tree, which tested various combinations different culture media evaluated use biodegradable polyester-based microvessels during adventitious rooting acclimatisation phases. Methods: Epicormic shoots were as explant source. After establishment multiplication, 8 BAP, NAA IBA elongation phase. Three types phase microcuttings. Results: had percentage 40.6% total 820 explants generated by 11th subculture, average 12 buds per explant. Best shoot results achieved BAP (0.05 mg L-1) + (1 combinations, whereas microvessel M2 M3 provided higher acclimatisation. According ISSR markers, at end 535 days cultivation, cloning was successful between acclimatised micro-plantlets parent plant. Conclusions: The efficient producing E. clonal recommended further studies species, testing other species.